Dissociates Clumped Cells in Minutes Results in Single Cell Suspension Gentle Cell Disaggregation for Maximum Cell Viability Yields Accurate, Reproducible Cell Counts Cost Effective Available Ready to Use Increase in cell count Various constructs of genetically engineered CHO cells, BHK cells, and a hybridoma were grown in suspension in serum-free or protein-free medium. Representative cell aliquots were treated with an equal volume of PBS or ACCUMAX and incubated for 5 minutes at 37°C. Cell number was then determined with a Coulter Counter.

has been developed to meet the most demanding needs for an effective cell aggregate dissociation solution. has been proven effective in dissociating clumped cells in suspension cultures of hybridomas, CHO, BHK, 293, and others. gently and rapidly dissociates cell clumps to yield single cell suspensions for accurate and reproducible cell counts. combines protease, collagenolytic, and DNase activities which maximizes its versatility for cell aggregate dissociation. does not contain mammalian or bacterial-derived products. is available as a ready-to-use liquid, quality controlled for in vitro cell culture applications.

Testing: Accumax has been extensively tested in research and biopharmaceutical laboratories.

Cell Lines: Accumax has been shown effective in dissociating cell clumps in a variety of cell lines including hybridomas, CHO, BHK, 293, COS and Sf9 cells.

Application: Accumax performs exceptionally well in dissociating cell clumps for cell counting, viral transfection assays, cell sorting, and flow cytometry as well as bioreactor scale-up.

Formulation: Accumax has protease, collagenolytic and DNase activity. Each lot of Accumax is individually formulated to meet quality control standards. Accumax is supplied in Dulbecco's PBS.

Availability: Accumax is available in ready-to-use 100ml bottles.