Detaches Adherent Cells in Minutes Gently Detaches hESCs Gentle Cell Detachment for Maximum Cell Viability Highest Plating Efficiency Cost Effective and Ready to Use Does not need to be aliquoted. Stable in the refer for two months. Cell Detachment Results CELL DETACHMENT: Human MG63 fibrosarcoma cells cultured on tissue culture treated dishes in DMEM + 10% FBS were treated with ACCUTASE. Treatment results in rapid cell detachment, a single cell suspension, and high viability. ACCUTASE is gentle on cells; viability was 97 ± 3% even after 45 minutes in ACCUTASE. Accutase allows a greater recovery rate of frozen hESCs hESC cultured in Invitrogen's Stempro hESC Serum and Freeder Free Media (SFM) were harvested either with Accutase or with Collagenase.  Harvested cells were frozen down respectively in the same condition.  After 2 month of Storage in LN2 , vials were thawed and plated respectively. After 1 day of recovery, phase images of the recovered cells, total cell number and viable cell number were obtained from respective group using a Countess automated cell counter.

has been developed to meet the most demanding needs for gentle and effective detachment of adherent cells. In the last two years, it has demonstrated outstanding performance with hESC cells. Download a scientific paper demonstrating Accutase's ability to grown hESC cells in a monolayer. hasalso been proven effective in detaching:fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, stellates, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080fibrosarcoma cells, and Sf9 insect cells. Download a list of recent publications using Accutase. does not need to be washed out or neutralized like trypsin; the addition of more media ineactivates Accutase, thus saving you much time in your cell passaging work. No more washes!! gently and rapidly lifts off adherent cells without destroying epitopes being measured with flow cytometry.. combines protease and collagenolytic activities which maximizes its versatility for cell detachment. does not contain mammalian or bacterial-derived products. is available in a ready-to-use formulation, quality controlled for in vitro cell culture applications.

Testing: Accutase has been extensively tested in research and biopharmaceutical laboratories.

Cell Lines: Accutase has been shown effective on: fibroblasts, keratinocytes, vascular endothelial cells, hepatocytes, vascular smooth muscle cells, hepatocyte progenitors, primary chick embryo neuronal cells, bone marrow stem cells, adherent CHO and BHK cells, macrophages, 293 cells, L929 cells, immortalized mouse testicular germ cells, 3T3, Vero, COS, HeLa, NT2, MG63, M24 and A375 metastatic melanoma, gliomas U251 and D54, HT1080 fibrosarcoma cells, and Sf9 insect cells.

Application: Accutase performs exceptionally well in detaching cells for: analysis of cell surface markers, virus growth assay, quiescence assays by serum starvation, transformation assays by oncogene transfection, neural crest cell migration assays, cell proliferation, cell haptotaxsis, tumor cell migration assays, routine cell passage, production scale-up (bioreactor), and flow cytometry.

Formulation: Accutase has both protease and collagenolytic activity. Each lot of Accutase is individually formulated to meet quality control standards for effective cell detachment. Accutase is supplied in Dulbecco's PBS containing O.5mM EDTA·4Na and phenol red.

Availability: Accutase is available in ready-to-use 100ml bottles & 500 ml bottles.